Title of the Training Program:

Advanced Training in Techniques of Epigenetic Regulation of Gene Expression

About training program:

The proposed training program aims to enhance research skills in the field of epigenetics and gene editing under the DBT-BUILDER program. The program includes hands-on training sessions that will guide participants on performing advanced techniques, such as Chromatin-immunoprecipitation (ChIP) assay, followed by quantitative real-time PCR (qPCR) to study specific gene promoter level enrichment of transcription factors and histone modifications. The three-day training session covers both the theoretical and practical aspects of the proposed assay, as well as the analysis of the obtained data. On the first day, participants will learn the fundamental principles and application of the assay system through research lectures by external and internal experts. Furthermore, on the same day, the participants will start the hands-on training by performing the key part of the ChIP assay, which will continue on the second day of the workshop. On the third day, ChIP-isolated genomic DNA will be used to perform qPCR analysis using unique gene promoter-specific primers. After completing the experiment, participants will also learn about the analysis of the acquired data. The main objective of the proposed workshop is to train young scientists in advanced techniques like ChIP assay and qPCR analysis, which will enable them to evaluate the promoter-level enrichment status of transcription factors, DNA binding proteins, and modified histones, indicating their role in regulating complex gene expression networks in both physiological and pathological contexts.